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Total protein extracts were obtained by homogenization of tissues using protein sample buffer [100 mM Tris-HCl (pH 6.8), 200 mM dithiothreitol, 4% sodium dodecyl sulfate (SDS), 0.2% bromphenol blue and 20% glycerol] and a classic protease inhibitor cocktail (Beyotime Institute of Biotechnology)

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Yoon, Hyoung Su Kim, Soung Won Jeong, Sung Eun Kim, JaeJun Shim, Yong Kyun Cho, Hyo Young Lee, Sung Won Han, Mindie H

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However, the deletion of HRG retarded the progression of MASLD-related liver carcinogenesis [44]
