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Informed Consent Informed consent was obtained from all individual participants included in the study

After nuclear labelling, cells were washed with 1xPBS, and the plates were stored with 100 l of PBS/well, protected from light at 4 C or taken directly to the Operetta automatic cell imaging system (PerkinElmer, MA, USA)

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A study found that kidney afferent nerves stimulate a brain-kidney neural circuit in CKD and heart failure, whichpromotes the SNS to increase disease progression in both organs
