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Multi-stage purificationincorporating techniques such as ultrafiltration, ion-exchange chromatography, and dialysisbecomes essential to achieve pharmaceutical-grade purity, as discussed in Section 4.3

It is thought that 2-HG may serve as an oncometabolite by impairing cellular functioning via a variety of epigenetic and metabolic mechanisms ( 2-HG is known to interfere with glutaminergic signaling and the functioning of -KG dependent enzymes, including those that are central to the regulation of hypoxia-inducible factor 1-alpha (HIF1) and vascular endothelial growth factor (VEGF) (34)

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10J and Additional file 11: Fig

[DOI] [PMC free article] [PubMed] [Google Scholar] 101.Bode K, Hauri-Hohl M, Jaquet V, Weyd H

References 1.Shaw R.J., Bradbury C., Abrams S.T., Wang G., Toh C.H