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The study by [49] shows that the concentration of secondary metabolites changes when different solvents are used

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Extracted samples were vortexed at 4 C for 10 min, cleared by centrifugation (17,000 g , 10 min, 4 C) and then supernatants were analysed by LCMS

It is an antioxidant It inhibits inflammation It improves insulin sensitivity, which lowers the risk of diabetes It is protective to the brain, and is thought to be a potential treatment for Parkinsons and Alzheimers It regulates the balance between regeneration and apoptotic cell shedding in the intestine

10.1093/toxsci/kfz055 22 FuL.ZhouL.GengS.LiM.LuW.LuY.et al (2020)

For bile salt tolerance testing, MRS broth was prepared containing bile salts at final concentrations of 0.1%, 0.2%, and 0.3% (w/v)