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The substrate binding by the SBD is implemented in two steps: after binding to the client protein via SBD-, HSP70s trap it by closing SBD-, serving as a cap or lid (Wang et al., 1998

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Adherence challenges of the ketogenic diet [A: 51:30, V: 58:00]

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Receptor Binding Assays Binding affinity is typically quantified using radioligand displacement or fluorescence polarization assays