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TGF-beta superfamily members from the helminth Fasciola hepatica show intrinsic effects on viability and development

Cells were seeded in 25 mL flasks (TPP Techno Plastic Products AG, Trasadingen, Switzerland) at an amount of approximately 2,400,000 cells/flask and incubated in standard conditions of temperature and humidity for 24 h before irradiation to allow their attachment

Peptides work at the cellular level by acting as signaling molecules that instruct cells to perform specific functions, such as repairing tissues, producing collagen, or regulating metabolic processes

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Pleurotus eryngii , Agaricus bisporus , and Flammulina velutipes have significant levels of ergothioneine, a sulfur-containing substance that protects cells

Whole-cell current was recorded with an Axopatch 200B amplifier and Clampex 10 software (Axon Instruments, USA)