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MMWR, 31(22), 290-291
Supernatants were subjected to SDS-polyacrylamide gel electrophoresis (4-15 %) in the presence and absence of 300 mM -mercaptoethanol (-ME)
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Retrieved 26 June 2021
The precipitate was then washed in once using PBS, centrifuged after blowing the precipitate well with a pipette gun, discarded the supernatant, and repeated once
