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The column was then washed with 5 mls of equilibration buffer before being washed with 3 5 mls of wash buffer (50 mM Tris, 300 mM sodium chloride, 20 mM immidazole, pH 8.0), before elution with 2 400 l of elution buffer (50 mM Tris, 300 mM sodium chloride, 250 mM immidazole, pH 8.0)

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In accordance with these studies, we assigned this to the oxidation of the sulphur moiety

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Glutathione S -transferase activities in mock- and GSTP1 -transfected HepG2 cells were in a similar range as reported previously in mock- (and GSTP1 -)transfected HepG2 cells (Peklak-Scott et al., 2008)