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doi: 10.1056/nejmoa1801445

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Kirsch R, Goldstein R, Tarloff J et al

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We first determined the rate constants associated (1) with the thiol-thioester exchange process involving peptide thioester 4 and MPAA ( k +2 , k 2 ), (2) with the conversion of SetCys peptide 2 into Cys peptide 3 ( k 1 ), and (3) with the reaction of peptide aryl thioester 7 with Cys peptide 3 ( k 4 ), from model reactions run separately (see Methods, Supplementary Tables 3 and 4)