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To date, no drug treatment has been approved against NAFLD, however, lifestyle alterations, physical exercise, and weight loss can regulate oxidative stress and the life cycle of mitochondria

Extracellular GSH was quantified by centrifugation and filter sterilization (VWR, 76479-016) of culture supernatants to remove cells

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By adding diamide and 2-DG simultaneously to LPS-stimulated cultured Ms and measuring NADPH over a defined time interval, we can assess NADPH consumption by GSR in a kinetic assay

Glutaminase inhibitors, such as CB-839, have shown promise in preclinical studies and early-phase clinical trials, demonstrating their ability to impede glutamine catabolism and disrupt the supply of glutamate, a precursor for numerous biosynthetic pathways critical for cancer cell survival and proliferation [12,55]

These findings indicate that the RIPK1 S32A mutation enhances the sensitivity of HCC cells to TNF-induced RDA, whereas the RIPK1 S32E mutation mitigates this sensitization