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[DOI] [PubMed] [Google Scholar] Schulz L

[DOI] [PubMed] [Google Scholar] 319

Mean fluorescence intensity (MFI) value was analyzed by the FlowJo V10 software

GST pull-down assays For GST pull-down assay, a tube of the 1 ml mouse liver cytosol was thawed on ice, centrifuged at 20,000 x g for 20 minutes at 4 C to remove any precipitated material, and the clarified cytosol was then diluted to a total protein concentration of approximately 20 mg/ml with cold homogenization buffer B (homogenization buffer A containing TX-100 at a final concentration of 0.1%)

Measurements were conducted at 20 C in 25 mM Tris-Cl pH 7.4, 25 mM NaCl, and 1 mM DTT

From these differentially expressed transcripts (DETs), DAVID software recognised 518 (Supplementary Table S4)