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Although we found that undiluted (i.e., 100% v/v) diacetyl (DA) is also highly toxic 26 , neither DA nor the strong repellent nonanone elevated the autofluorescence signal (Supplementary Fig

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However, the activity reported was very low, which could be enhanced to 963-fold for a triple mutant E423Y/E442Q/D445N of BsGGT, developed by site-directed and random mutagenesis approach (Suzuki et al., 2010)
