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Not recommended beyond this

Following the specified treatments, cells were collected and lysed and resulting global protein abundance was analyzed using LC-MS/MS

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The third vessel, the sailing ship San Jos , left New Spain later that spring but was lost at sea with no survivors

That its counterintuitive

As controls, we used Man1A1-GFP, a Golgi membrane protein with its C-terminal in the lumen (proteinase K resistant), and CTNS-GFP, a lysosome membrane protein with the C-terminus facing the cytosol (proteinase K sensitive)