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Cells were then resuspended in a staining buffer [PBS with 0.5% BSA, 2 mM EDTA, 100 g/mL RNAse (Sigma Aldrich, Burlington, MA, Cat#: 10109142001) and propidium iodide (BioLegend, Cat#: 421301)] and incubated for 1 h at 4C

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10.1111/add.12850 [DOI] [PMC free article] [PubMed] [Google Scholar] Kang H., Xiao X., Huang C., Yuan Y., Tang D., Dai X., et al

Riboflavin deficiency causes protein and DNA damage in HepG2 cells, triggering arrest in G1 phase of the cell cycle