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Beyond its vascular benefits, Adropin exerts metabolic effects that may alleviate BBB damage

The first uptake is of glutamate and GABA into astrocytes, where a large fraction is converted to glutamine in an energy-requiring process, and the second uptake is that of glutamine into neurons

10.1002/ijc.24988 Int

Arch Physiol Biochem 129(1):6168

Data availability All data associated with this study are present in this paper or the supplementary information

10% (w/v) tissue homogenates were prepared in ice-cold isolation buffer with 1.0 mM ethylene glycol-bis(-aminoethyl ether)- N , N , N , N -tetraacetic acid (EGTA), [215 mM mannitol, 75 mM sucrose, 0.1% fat-free bovine serum albumin (BSA), 20 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (HEPES), 1.0 mM EGTA and pH is adjusted to 7.2 with potassium hydroxide (KOH)]