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Cells were resuspended in 5 to 10 mL of lysis buffer (20 mM sodium phosphate pH 7.5, 200 mM NaCl, 0.05% (w/v) Tween-20, 2 mM MgCl 2 , 0.5 mM TCEP, +400 U DNAse I, 12.5 mM imidazole and a tablet of Complete EDTA-free protease inhibitor cocktail, Roche, per 100 mL of buffer) per gram of cells

Basu, T

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10.1007/s00280-012-2047-z 30 Boyette-DavisJ.DoughertyP

Jones et al

31,32 Four double-blind, placebo-controlled trials evaluated the efficacy of zinc alongside methylphenidate (Ritalin), and found that children in the zinc group improved more than those in the placebo group, 33,34,35,36 although this was not statistically significant in one study