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The hippocampal tissue was disrupted using a T10 Ultraturrax homogenizer (IKA Werke GmbH) followed by RNA extraction with TRIzol Reagent (Invitrogen, Thermo Fisher Scientific) according to the manufacturers instructions

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investigated heat-stressed laying hens and found that STAR , CYP11A1 , and 3HSD expression in granulosa cells increased initially, while FSHR and CYP19A1 expression decreased under heat stress conditions [83, 84]