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THP-1 cells were differentiated into macrophages by 100 nM of phorbol-12-myristate-13-acetate (Sigma-Aldrich), and differentiated into M1 by adding LPS (100 ng/mL, Sigma-Aldrich) and IFN- (20 ng/mL, R&D Systems) for 24 h, and M2 cells with M-CSF (20 ng/mL, Peprotech), IL-4 (40 ng/mL, R&D Systems), and IL-13 (40 ng/mL, R&D Systems) for 4 days

Always consult with a qualified healthcare provider before beginning any new supplement, peptide, or research compoundespecially those with central nervous system activity or off-label applications

71,72 5 What Causes Tinnitus

The reversed spike-timing-dependent plasticity acts as sensory filtering

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