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We report the novel observation that GSTP1 is modified by the palmitate analogue -alkynyl-palmitate (Fig 1A, top panel) as evidenced by the detection of a neutravidin-HRP signal (revealing the presence of a GSTP1-palmitate-biotin complex)

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With the monitoring of the temperature and ventilation during the processing, the highest yield of 206 7 mg/l (17.34 mg/g) of erinacine A could be obtained after 14 days of cultivation using a 100 l bioreactor with the medium containing 0.5% yeast extract, 4% glucose, 0.5% soybean powder, 0.25% peptone, 1% oat, and 0.05% KH 2 PO 4 at pH 5 [30]

The spectra quality requirement was full width half height linewidth less than 2.5 x standard deviation (SD) above the group mean

The strong association of anti-Jo-1 levels with clinical outcomes suggests that these autoantibodies may have a direct pathogenic role in IIM that is mitigated by B cell depletion with rituximab and may be a good biomarker for disease activity