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E., Amaral S

Male: There is no clear effect on seminal proteins and we anticipate indirect alterations in spermatogenesis

Injections are strictly not recommended

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FT-IR spectra were recorded using a Thermo Scientific NICOLET 6700 optical spectrometer (Waltham, USA), following the procedure described by 1 at a controlled temperature of 25 C, and the characteristic absorption peaks were analyzed to identify the functional groups present in the extract

Cell lysis for protein preparation The yeast cell pellet in a 1.5-mL tube (on ice) was resuspended with 500 L ice-cold lysis buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl, 1.8 mM MgCl 2 , 0.5% NP-40, 1 mM PMSF, 1 mM DTT, 20 M MG132, 1 tablet/10 mL cOmplete mini EDTA-free (Roche)) and transferred to a new 1.5-mL tube containing 50 L of 0.5 mm Zirconia/Slica Beads (BioSpec Products)