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In accordance with this, it has been estimated that the steady-state concentration of superoxide in the mitochondrial matrix is 5- to 10- fold higher than in the cytosol (Fernandez-Checa and Kaplowitz, 2005
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Flow cytometry Flow cytometry (CytoFLEX, Beckman Coulter, Inc., Miami, FL, USA) was used to measure apoptosis and ROS levels in testicular cells following a previously described method [54]
L., Sinnard, M
