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As soon as the supernatant was centrifuged, 400 mL of the supernatant was poured into the corresponding EP tube and stored overnight at 20 C in the refrigerator before being centrifuged at 12,000 r/min for 3 min at 4 C, and approximately 2000 mL of supernatant was collected in the injection bottle liner for on-board analysis

The CARS is a 15-item clinician-rated behavioural scale that integrates caregiver reports with direct clinical observation

Effects of artificial light at night on human health: A literature review of observational and experimental studies applied to exposure assessment