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To better understand the mechanism, the authors measured levels of reduced glutathione (GSH) and oxidized glutathione (GSSG) and calculated a GHS/GSSG ratios (with a decreased ratio indicating significant oxidative stress) [52]

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The homogenate was centrifuged for 20 min at 12,000 g and the supernatant obtained was used for enzyme analysis

This paper aims to summarize recent advances in non-pharmacological treatments for AD, including physical therapy, exercise therapy, cell therapy, and nanoparticles (NPs), and to discuss the research progress and challenges associated with these approaches