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An autoregulatory negative transcription-translation loop feedback system involving cyclical synthesis of the period proteins Per1-3, Clock/BMAL1, and Cry1/2 acts as the basic molecular oscillator, where the Clock/BMAL1 heterodimer acts to activate the transcription of Per and Cry proteins (the so-called positive limb)

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Depending on the compound being reconstituted and the intended use, a reconstitution solution could be bacteriostatic water, sterile water, sterile saline, or even a specially buffered solution designed for a particular peptide or medication

Is it a downstream cascade effect initiated by that initial brief pulse, or is another variable at play