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doi: 10.1186/s12974-019-1528-y 87

The freeze-thaw cycle can damage the peptide's structure through the formation of ice crystals, leading to aggregation and loss of bioactivity

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Postharvest Biol Tec 86:431436 Blanco NE, Guinea-Daz M, Whelan J, Strand (2014) Interaction between plastid and mitochondrial retrograde signalling pathways during changes to plastid redox status