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history taking, clinical examination, validated IIEF-5 questionnaire, estimation of serum l-carnitine by calorimetric method and serum 25(OH)D by ELISA method
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These procedures involve the use of a minimally invasive surgical technique: a small incision is made to access the kidney or upper ureter with a nephroscope, which is then used to fragment (nephrolithotripsy) or remove (nephrolithotomy) the stone (Vicentini 2009
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