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MEF cell culture Control, Bmal1 / and Cry1 / Cry2 / MEFs were cultured in DMEM (Sigma-Aldrich) supplemented with 15% FCS (Atlanta Biological) and 1% penicillin-streptomycin at 37 C with 5% CO 2

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Specifically, germ-free mouse models or FMT can be adopted to confirm a causal chain that iron overload mediates a reduction of gut Lactobacillus , which in turn aggravates the development of OA

J Elem 25:549563

Tumor-associated macrophages (TAMs), important immunosuppressive cells in the microenvironment, are able to activate the Gli2/IGF-2/ERK1/2 signaling axis to promote TGF- secretion and thus mediate the migration, invasion and EMT of hepatocellular carcinoma cells (Huh-7 cells) (73)
