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LC-MS/MS-based proteomics Sample collection and protein isolation Neurons were collected on ice in phosphate-buffered saline (PBS: 137 mM NaCl, 2.7 mM KCl, 7.3 mM Na 2 HPO 4 , 0.9 mM CaCl 2 and 0.5 mM MgCl 2 , pH 7.4) with protease inhibitor (Complete tablets, Roche)

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Follow the recommended duration of use, as specified by the product or as advised by your healthcare professional
