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Briefly, cell pellets or tissues were homogenized in 1 ml TRIzol Reagent (Ambion, Life Technologies, CA), total RNAs were separated from other cell contents by sequential chloroform and isopropanol precipitation

At the end of the reaction a fine homogeneous powder is obtained (Jasim et al., 2020

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Epigenetic and metabolic reprogramming in inflammatory bowel diseases: Diagnostic and prognostic biomarkers in colorectal cancer