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A treatment with epigallocatechin gallate (EGCG), a polyphenol from green tea, restored microbial composition, preserved tight junction expression, and reduced systemic inflammation (Yang J.-Z

[DOI] [PMC free article] [PubMed] [Google Scholar] 194.Wu J., Shao Y., Liu J., Chen G., Ho P.C

doi: 10.1101/cshperspect.a021109

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Peptide digests were cleaned by solid phase extraction (SPE) using Strata-X 33 m polymeric sorbent cartridges (Phenomenex, 30 mg/1 mL, #8B-S100-TAK), as per the manufacturers instructions, with final elutes using sequential addition 70% MS-grade acetonitrile (ACN) with 0.5% FA and 90% MS-grade ACN with 0.5% FA
