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The earliest six fractions containing significant DiD-C18 fluorescent signal were combined and concentrated in a 30 kDa Airmcon Ultra Centrifugal filter for protein characterization by BCA, SDS-PAGE, and MS Proteomics, in addition the UV-1600PC, UV/Visible Spectrophotometer (VWR) were used to quantify LNP total lipid content by DiD-C18 absorbance ( Max 649 nm) and molar extinction coefficient (260 000 cm -1 M -1 )
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