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Group separation in a valid PLS model resulted in 64 NMR and 3568 significant MS features, where 43 NMR and 1986 MS features retained differential significance from D5 to D9 after T-test and fold change filtering (Supplementary Fig

[4] Almannai M, Alfadhel M, El-Hattab AW

These microbial shifts coincide with elevated SCFA levels and increased expression of UCP1, CPT1B, and SIRT1 in adipose depots, indicating that AX promotes thermogenesis through a gut microbiotametaboliteUCP1 regulatory axis that integrates intestinal and systemic energy homeostasis [73,75,76]
The mass spectrometer was configured in multiple reaction monitoring mode, and the monitored transitions for carnitine were m/z 162.143.2 and 162.160.2

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