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After cell counting, the cells in each group were centrifuged at 210 x g for 5 min at 37C to remove the supernatant and then diluted in PBS to 5x106 cells/ml

A 2014 study in the European Journal of Obstetrics and Gynecology and Reproductive Biology demonstrated that women with PCOS who were resistant to clomiphene (a prescription drug that can help a woman become pregnant), were more likely to become pregnant when 3,000 mg of L-carnitine was added to their daily drug regimen

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Tissue sections were observed under a Leica DMI-3000B microscope (Leica, Germany)

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