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As the quality of kefir can be driven by factors such as fermentation temperature, time, types of kefir grains, substrate availability, more research studies on kefir production are needed to further validate and determine the exact parameters for producing a consistent and standard kefir

For instance, compounds like kojic acid show promising inhibitory effects but face limitations due to cytotoxicity or allergic reactions

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Cell pellets were collected and resuspended in lysis buffer (50 mM Tris pH 8.0, 200 mM NaCl, 5 mM -mercaptoethanol, 10% glycerol, and 1X Halt protease inhibitor (Thermo Fisher), and frozen overnight at 80 C