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3.Sikiric

For immunofluorescence staining, samples were immersed in 1% paraformaldehyde (Nacalai Tesque) overnight at 4C, embedded in OCT compound (Sakura), frozen, and then sectioned

The mixture was then centrifuged at 13,000 r/min for 15 min at 4 C, and 200 L of the supernatant was transferred to injection vials for UPLC/Q-TOFMS analysis

Exercise-associated myokines such as irisin (Ciaowicz et al., 2025) and IL-6 (Mao et al., 2025) are linked to improved hepatic insulin sensitivity and mitochondrial performance, whereas catabolic signals released during sarcopenia, including myostatin, are associated with impaired hepatosteatosis and mitochondrial dysfunction (Joo and Kim, 2023)

Add bacteriostatic water recommended volumes are 1 mL for 10 mg vials, 2 mL for 40 mg vials, and 2.5 mL for 50 mg vials

De Meij et al., 2021