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Role of miR-125b-5p in modulating placental SIRT7 expression and its implications for lipid metabolism in gestational diabetes

2.2 RNA isolation and real-time polymerase chain reaction (PCR) analysis The total RNA was isolated from the heart apex tissue using Tri reagent (Sigma-Aldrich)

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Dynamic alterations in the density of genomic methylation patterns can lead to dynamic changes in gene expression in response to various internal or external factors

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