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Description
The damaging effects of proinflammatory CK were described and characterized in rat [66] or cARLA treated human BBB models [40]
Endogenous peroxidase activity was blocked with 3% hydrogen peroxide (HO) for 10 min, followed by antigen retrieval in 0.1 M citrate buffer (pH 6.0) heated to 95 C for 15 min, then cooled at room temperature for 30 min
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Similarly, in rat hepatic stellate cells (HSCs) TGF-1 suppressed the expression of GCLC (no effect on GCLM) and lowered GSH levels [73]
The results are long-lasting
