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doi: 10.1038/nchembio.2007.33

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The transfection complex was formed at a DNA:PEI ratio of 1:3 in OPTI-MEM (Life Technologies, Paisley, UK), with a 30 minute incubation at room temperature prior to addition to the cells

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Furthermore, there was a significant reduction in the levels of the proinflammatory cytokine IL-6 in both the plasma ( Figure 4A ) and PBMCS ( Figure 4B ) from T2DM subjects who received L-GSH supplement