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If no, go to step iv

IPL to clear accumulating sun damage

Then the cells were permeabilized with blocking/permeabilizing solution (50 mM NH 4 Cl, 0.1% Saponin, 1% BSA in PBS), and an Alexa568-conjugated secondary antibody was used to label endocytosed anti-PrP antibody bound to its ligand

-20C, 1 month (protect from light)

Residual peptide may still be absorbing through the sublingual tissue, and food or drink can interrupt this process

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