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Additionally, patients could worsen during the study and still be classified as recovered and the study was unblinded with subjective outcomes

These studies have primarily utilized mouse models of immunosuppression, often induced by the chemotherapeutic agent cyclophosphamide (CTX), which depletes immune cell populations

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Holbrook-Smith D, Durot S, Sauer U

Fifteen g of total protein extract from gills were diluted 1:1 with 2X Laemmli Sample Buffer (Bio-Rad) containing 100 mM dithiothreitol (Sigma-Aldrich)

Rizkallah et al., 2010