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After treatment, the cells were collected and co-incubated with BMDCs at a 1:1 ratio for 12 h

DNA purity was assessed using a NanoDrop 2000/2000c spectrophotometer (Thermo Fisher Scientific), while DNA concentration was determined with the Qubit Fluorometer (Life Technologies, Monza, Italy) using the Quant-iT dsDNA High Sensitivity Assay Kit

[PubMed] [Google Scholar] 84.Chiodetti L and Schwartz RH, Induction of competence to respond to IL-4 by CD4+ T helper type 1 cells requires costimulation

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Orbitrap-based ultra-high-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS) in data-dependent acquisition (DDA) mode was utilized to detect both phase-I and phase-II reaction products, together with in silico prediction and a home-made HbS-PI biotransformation database for in-depth metabolite screening

However, there is still no clear evidence if and how acetylcarnitine supplementation alone can improve neuropsychiatric symptoms of long COVID