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Vitamin B12 is a water-soluble vitamin that plays an essential role in many bodily functions, including the metabolism of carbohydrates, fats, and proteins

Untargeted profiling of the metabolome is performed using label-free analytical techniques, MS and NMR-based approaches being the most popular (Kuehnbaum & Britz-Mckibbin, 2013)

Dobson et al., 2013

These data suggest that the anticoagulant used for sample collection and time to deproteinization, both independently and additively impact measured levels of free GSH (and thus the ratio of free GSH to GSSG) in samples of plasma which are deproteinized and stored at 80 o C

The results of this study indicated that ACA, as a soft matrix, exhibited the lowest in-source fragmentation in the analysis of heat-sensitive molecules like gangliosides