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For protein extraction, worm pellets were homogenized via bead beating in non-denaturing lysis buffer (100 mM HEPES pH 7.4, 300 mM NaCl, 2 mM EDTA, 2% Triton X-100) supplemented with EDTA-free protease inhibitor and PhosSTOP phosphatase inhibitor cocktails (Cat

It is still unclear if HCV genotypes originated from a single cross-species transmission and subsequently diverged within the human host or from separate zoonotic sources in different regions 5

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