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pulse on 4 s, pulse off 6 s, total time 40 min), and cell debris were removed by centrifugation (12000 g , 2 min, 4C)

Then, samples from the basolateral side are taken at different time points

GSH degradation results in sulfur reallocation and is believed to be carried out mainly by glutamyl cyclotransferases (GGCT2;1, GGCT2;2, and GGCT2;3), which, however, do not fully explain the rapid GSH turnover

doi: 10.1186/s12966-023-01461-x 93

Detoxification Glutathione plays a key role in liver detox, helping eliminate toxins and heavy metals from the body