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Intracellular reactive oxygen species (ROS) determination After different pretreatments, a ROS assay kit (S0033S, Beyotime, China) was used to determine the ROS levels in the GC cells

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This mechanism leads to decreased expression of hypoxia-inducible factor (HIF)-1 and Tf while boosting the levels of proteins such as FTH1, NAD(P)H quinone dehydrogenase 1 (NQO1), HO-1, and GPX4

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