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The extract and cells were scraped and collected into 2 ml EP tubes, held at 4 C for a further 5 min and centrifuged for 5 min at 12,000 g and solvent in the resulting supernatant was evaporated using a speed-vac

doi: 10.1007/s13238-020-00718-6

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Ligand-directed signalling within the opioid receptor family

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The Bcl-2/Bax ratio is suggested to play a potential role in the regulation of apoptosis