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Preclinical studies have demonstrated that over time, cells continuously treated with ADCs eventually show a decrease in the expression of target antigen proteins (such as CD30) and other effects ( As a key factor in the effective design of ADCs, DAR refers to the average number of drug molecules linked to each antibody ( As for the toxic events that often occur in ADCs, such as hepatotoxicity, they are often related to their specific payloads

Liu S, Tsui MT, Lee E, Fowler J, Jia Z

Nutr Cancer 2016;68:117

For enzyme extraction 60 mM NaP (pH 6.1) with 0.5 mM EDTA was used [33]

Shima et al., 1991

Effects of riboflavin deficiency in rats on some aspects of iron metabolism