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Immunofluorescence assay SK-MEL-147 cells were collected and seeded into the inner 60 wells of 96-well, black-walled plates (Agilent, 204626-100) at 1000 cells per well in 100 uL of complete DMEM overnight in a 37 C incubator with 5% CO 2

Shoulder injections to treat rotator cuff injuries typically target the small space between 2 bones: The bottom of the acromion (the top outer edge of the shoulder blade) The top of the shoulders ball-and-socket joint (glenohumeral joint) This space is called the subacromial space and the injections are called subacromial injections

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Storage and stability after reconstitution You have mixed your vial perfectly

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