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Microautophagy includes autophagic tubes directly engulfing the cytoplasmic components [71]

Cell proliferation induced by GPE was further delineated by an increased level of phosphorylated Akt, indicating potential upregulation of the ERK ( Figure 6 ) and PI3K/Akt signaling pathways ( Figure 7 ) pathways, which are among the signaling pathways involved in the regulation of cell proliferation

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Subjects with known clinically relevant comorbidity, acute illness, serious infectious disease for 4 weeks prior the product intake, abnormal screening laboratory tests, and abnormal blood pressure (systolic blood pressure 139 mmHg and/or diastolic blood pressure 89 mmHg) were excluded, although subjects with normal blood pressure taking anti-hypertensive medication were included

At the end of the administration, we sacrificed the rats by intraperitoneal injection of 3% pentobarbital sodium (100 mg/kg), and the tissue samples were processed differently according to the different experimental protocols