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Reverse transcription and real-time PCR Total RNA (1 g), isolated from cells using PerfectPure RNA Cultured Cell Kit (5 Prime) was reverse transcribed using random hexamers and Superscript II RNase H Reverse Transcriptase (Invitrogen)

Homocysteine metabolism involves two pathways: remethylation, where homocysteine is converted back to methionine using a methyl group from N5-CH3-TH4, completing the methionine cycle

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These combination approaches may enhance specific research outcomes[10]